On Monsieurs Departure - 1190 Words

On Monsieur s Departure, with its highly interpretive nature and use of strong themes and appropriate literary devices, expresses the inner turmoil of its author, Queen Elizabeth, to the reader. The basic concept of this 17th century poem is one of the divided passions of Queen Elizabeth. Elizabeth loved her country with fierce loyalty and control, but also had her own personal needs, and though it is not entirely certain as to whom this poem was referencing to, it is speculated to be about either the 2nd Earl of Essex or the Duc d Anjou (French duke of Anjou). Essex (Robert Derereaux) was 30 years Elizabeth s junior and was a charming, opinionated man with whom Elizabeth was completely enamored, but the relationship terminated when†¦show more content†¦The line, Follows me flying, flies when I pursue it, with use of alliteration is concise and artful, with a condense accumulation of emotion. The theme of Elizabeth s care (of this man) being unattainable for her to grasp is very eminent in this stanza, with two supporting examples. The first comes in lines 7 and 8, where Elizabeth conveys, My care is like my shadow in the sun,/Follows me flying, flies when I pursue it. She is articulating that her own personal feelings are unconstrained to her, without the control she is wishing to enforce. The second instance in line 11, No means I find to rid him from my breast, expresses not that there is a physical person which she feels the need to remove, but instead that Elizabeth believes that there is no way to eradicate the thoughts and feelings she has for this man from her heart. This continuing theme of separation from oneself and separation from emotional control is echoed throughout the poem, creating a delicate symmetry between each stanza, and allowing them to unite. The third stanza of On Monsieur s Departure embraces again figurative language, repetition, and a final accumulation of sentiment through deliberative wording. In this stanza, Elizabeth uses a very important metaphor (expression relating one thing to another) to represent herself. She writes, in line 14, that she is made of melting snow, a phrase which captures thoughts and images that may otherwise not have been soShow MoreRelatedMonsieurs Departure853 Words   |  4 PagesOn Monsieur’s Departure This poem is one of the nicest poems, which was written by Queen Elizabeth I. Her poems, speeches and translations illustrate her political genius and her rhetorical ingenuity. The â€Å"On Monsieur’s departure† is a poem that talks about love. Queen Elizabeth I fell in love, but because of her position she couldn’t reveal her true feelings. She couldn’t decide what to do and at the end she was very frustrated because unfortunately she had to ignore her feelings because ofRead More Comparing Love and Marriage in Canterbury Tales, Lanval, Faerie Queene, and Monsieurs Departure675 Words   |  3 PagesLove and Marriage in Canterbury Tales, Lanval, Faerie Queene, and Monsieurs Departure Medieval and Renaissance literature develops the concepts of love and marriage and records the evolution of the relation between them. In Chaucers Canterbury Tales, Christian love clashes with courtly love, as men and women grapple with such issues as which partner should rule in marriage, the proper, acceptable role of sex in marriage, and the importance of love as a basis for a successful marriage. WorksRead MoreEssay on Queen Elizabeth I in Love922 Words   |  4 PagesElizabeth declares herself the judge of a wager to see if a play can capture the essence of true love. In order to judge that wager accurately she has to have knowledge of love. Her love, and misery, is beautifully described in her poem On Monsieur’s Departure. In it she describes her love for the Duke and her misery in being forced to deny this love. I love and yet am forced to seem to hate, / I do, yet dare not say I ever meant†¦ She loves him passionately, but denies that love in order to protectRea d More Shakespeare In Love Essay example985 Words   |  4 Pages good, and safety whereof I will never shun to spend my life. (Elizabeth I 1028) Though she fulfills her promise, she endures heartbreak as the result. Unable to show her pain to her subjects, Elizabeth resorts to poetry. In her poem, On Monsieur’s Departure, which predates Romeo and Juliet, she resembles both Viola, and Shakespeare’s Juliet. Regarding Duke d’ Alencon, she writes, I am and not, I freeze and yet am burned, / Since from myself another self I turned. (Elizabeth I 1024) ClearlyRead Moreâ€Å"the Spirit of Renaissance and Elizabethan Era†5448 Words   |  22 PagesEnglish musicians of the time, and are often seen as being a part of the same artistic movement that inspired the above authors. Elizabeth herself, a product of Renaissance humanism trained by Roger Ascham, wrote occasional poems such as On Monsieur’s Departure at critical moments of her life. English Renaissance theatre English Renaissance theatre, also known as early modern English theatre, refers to the theatre of England, largely based in London, which occurred between the Reformation and the

Analysis of Frederick Douglas Narrative of a Slave Essay

In the Narrative of the Life of Frederick Douglass, an American Slave, Written by Himself, Frederick Douglass establishes for us the many factors that lead to the continual enslavement of the black race by connecting his own plight to that of other slaves as a plea for the abolition of slavery. The evil of slavery infected every master to pervert the truth to his own satisfaction and Douglass explains how slavery corrupts the humanity of both slave and master. The legal system was also not an option for slaves to turn to for help because they had no legal rights. The fear of losing friends and never being able to trust anyone again was enough to keep many back in bondage. And the lack of education left their minds dulled to any†¦show more content†¦Douglass assures us that just the opposite is the truth. From the great big house of the plantation owner all the way down to the fields where the slaves toiled, all was in harmony according to the master`s ideal. Any show of di scontent was sure to lead to a brutal whipping or other punishment because nothing was to deter from the portrait of a picture perfect institution. Douglass also emphasizes that the evil power of slavery will turn a good, kind heart, stone cold as it did with his masters wife, Mrs. Auld. She was once loving to him and wanted to educate him, and felt uncomfortable around his servility. But as time went on and she listened to her husbands demands to keep Frederick uneducated, the power she had over him turned her into a cruel master as well. Douglass believes that if a master isnt at the start cruel and mean, the power he or she has over another person will corrupt them and turn them so. The evil of slavery affects not just the slaves who suffer under its weight but it makes the slave owner cold and cruel. So slavery could not be quelled with kinder masters because in time they would become just as harsh as any other. Because slaves were classified as inferior and not quite human, the legal system judged them as less credible than their masters. In the courts, the testimony of a black witness was never equal to that of a white witness.Show MoreRelatedThe View from the Bottom Rail Essay703 Words   |  3 PagesRail   In today’s society, racism and stereotyping occur in every aspect of life. No one should ever take anything for face value before they examine it first.  In reading the narrative, â€Å"The View from the Bottom Rail† by James Davidson and Mark Lytle and â€Å"Frederick Douglasss Narrative of the Life of Frederick Douglas† by Harold Bloom. I became very aware on how American history can be looked at as one sided or bias. Even in today’s society, there is still a lot of biasness presented in AmericanRead MoreJacobs Douglass: An Insight Into The Experience of The American Slave1019 Words   |  5 PagesThe slave narratives of the ante-bellum time period have come across numerous types of themes. Much of the work concentrates on the underlining ideas beneath the stories. In the narratives, fugitives and ex-slaves appealed to the humanity they shared with their readers during these times, men being lynched and marked all over and women being the subject of grueling rapes. The slave narrative of Frederick Douglas and Harriet Jacobs: Incidents in the Life of a Slave Girl themes come from the existenceRead MoreKey Arguments in The Narrative of the Life of Frederick Douglass1154 Words   |  5 Pagesnumber of key arguments in â€Å"The Narrative of the Life of Frederick Douglass†. A few of which include inequality, education, and Christianity as the keys to freedom in terms of its true values within the institution of slavery. While Frederick Douglass made some key arguments, he also made common ground to make his appeal for the abolition of slavery. One of the key arguments in â€Å"The Narrative of the Life of Frederick Douglass† as well as in other narratives about slaves is inequality. Douglass attemptsRead MoreFrederick Douglass: Literary Analysis Essays1243 Words   |  5 Pagesâ€Å"Narrative Life of Frederick Douglass: Literary Analysis† In Narrative of the Life of Frederick Douglass, Douglass explains, in great detail, how slave master would use a variety of methods to dehumanize slaves located on their plantation. These methods involved both severe physical and psychological trauma. Nevertheless, Douglass remains diligent and finds a way to resist the harsh reality of being a slave. Because of his immovable desire to acquire knowledge to his fighting encounter with MrRead MoreEnslavement Disrupted the African’s Authentic Culture Essay1593 Words   |  7 Pagesthem in America as slaves. As a result, their authentic cultures were drastically changed from the way of life in their native Africa to life in the plantation society of the Americas. In this essay, I will attempt to show how the conditions of enslavement disrupted all dimensions of the African’s authentic culture. To aid in my analysis, I will be using the â€Å"Reid Culture Conflict Model† as a guide and also drawing upon the works o f Olaudah Equiano, Venture Smith, Frederick Douglas, Harriet JacobsRead MoreThe Plight of The African Slave Essay1558 Words   |  7 PagesMiddle Passage many slaves perished. Those who survived were sold and subjected to the harsh life on the plantations. When this happened, their authentic cultures were drastically changed from the way of life in their native homelands in Africa to life in the plantation society of the American colonies. In this essay, I will attempt to show how the enslaved Africans’ authentic culture was immensely disrupted by using the â€Å"Culture Conflict Model† as a guide. To aid in my analysis, I will be drawingRead MoreThe Narrative of the Life of Frederick Douglass1527 Words   |  7 Pagesracist society back in those days. African talents were absolutely wasted and they were considered inferior to white individuals. The â€Å"Narrative of the Life of Frederick Douglass† simply addresses that white society was causing negativ e effects to itself and that slavery must be abolished in order to shape a better world. The depicted brutality narrated in Frederick Douglass’s autobiography starts when Douglass states that his former overseer, Mr. Plummer, â€Å"cuts and slashes the women’s heads so horriblyRead MoreThe Life Of Frederick Douglass Context1480 Words   |  6 PagesBehind Frederick Douglass context, Douglass was a former slave who became one of the most successful abolitionist of 19th century. With his oratorical and literacy appeals, in 1852, Douglass delivered a speech that changed the views of millions over the Fourth of July. By using the experience Douglas has encountered as a slave, the historical context to support his argument and certain rhetorical devices, Douglass remarks a speech in order for the leading citizens of Rochester to be aware of theRead MoreThe Life Of Frederick Douglass Context1479 Words   |  6 PagesIntroduction Behind Frederick Douglass context, Douglass was a former slave who became one of the most successful abolitionist of the 19th century. With his oratorical appeals, in 1852, Douglass delivered a speech that changed the views of millions over the Fourth of July. By using the experience Douglas has encountered as a slave, the historical context to support his argument and certain rhetorical devices, Douglass remarks a speech in order for the citizens of Rochester to be aware of the hypocrisyRead MoreAn Analysis of the Narrative of the Life of Frederick Douglas757 Words   |  4 PagesAn Analysis of the Narrative of the Life of Frederick Douglas In the autobiography Frederick Douglass presents a clear picture to me of a horrifying period of American history that far too few people understand. Douglass’s personal narrative as a slave lets you feel the fear of his past and allows us to experience the suffering and pain inflicted by underserved beatings and an unhealthy lifestyle with too much physical exertion. Douglass expresses very personal feelings about his history and

Rockwells Illustrations Essay Example For Students

Rockwells Illustrations Essay In America, artists works are not only shown in museums, they are often displayed on magazine covers. Norman Rockwell produced cover paintings for the Saturday Evening Post, a major magazine of the 1910s and for many decades later. In the process he became a nationally renowned artist. His precise detail brought him great popularity. He created a moral myth in which people were reassured of their own essential goodness, art critic Arthur C Danto told Allison Adato of Life magazine. And that is a very powerful thing. Film director Steven Spielberg remarked to Adato, Growing up, we always subscribed to the Post. He saw an America of such pride and self-worth. My vision is very similar to his, for the most part because of him. When people use the expression as American as apple pie they could just as well say as American as a Norman Rockwell painting. Rockwell was born on February 3, 1894, in New York City. His father worked for the textile firm, starting as office boy and eventually moving up to manager of the New York Office. His parents were very religious and the young Rockwell was a choir boy. Until he was about ten years old the family spent its summers in the country, staying at farms. Rockwell recalled in his autobiography My Adventures as an Illustrator. I have no bad memories of my summers in the country, and noted that his recollections all together formed an image of sheer blissfulness. He believed that these summers had a lot to do with what I painted later on. Rockwell enjoyed drawing at an early age and soon decided he wanted to be an artist. During his freshman year in high school, he also attended the Chase School on Saturdays to study art. Later that year he attended Chase twice a week. Halfway through his sophomore year, he quit high school and went full time to art school. Rockwell enrolled first in the National Academy School and then attended the Art Students League. Because he was so dedicated and solemn when working at his art, he related in his autobiography, he was nicknamed The Deacon by the other students. In his first class with a live model, the location of his easel was not the best. The nude young woman was lying on her side and all Rockwell could see was her feet and her rear end. So that is what he drew. Rockwell noted that, as Donald Walton wrote in his book A Rockwell Portrait, He started his career in figure drawing form the bottom up. At the Art Students League, Rockwell had two teachers who had a significant influence on him: George Bridgeman, a teacher of draftsmanship, and Thomas Fogarty, a teacher of illustration. Besides their expert instruction, Walton wrote, they conveyed their enthusiasm about illustration. While still at school, Fogarty sent Rockwell to a publisher, where he got a job illustrating a childrens book. He next received an assignment from Boys Life magazine. The editor liked his work and continued to give him illustration assignments. Eventually Rockwell was made art director of the magazine. He regularly illustrated various other childrens magazines after that. I really didnt have much trouble getting started, he remarked in his autobiography. The kind of work I did seemed to be what magazines wanted. In March of 1916, Rockwell traveled to Philadelphia to attempt to see George Horace Lorimer, editor of the Saturday Evening Post, to show him some proposed cover paintings and sketches. It was his dream to do a Post cover. So he set out to sell Lorimer on his work. Since he did not have an appointment, the art editor came out and looked at his work, then showed it to Lorimer. .u6872f86bc330512477712e5edee69319 , .u6872f86bc330512477712e5edee69319 .postImageUrl , .u6872f86bc330512477712e5edee69319 .centered-text-area { min-height: 80px; position: relative; } .u6872f86bc330512477712e5edee69319 , .u6872f86bc330512477712e5edee69319:hover , .u6872f86bc330512477712e5edee69319:visited , .u6872f86bc330512477712e5edee69319:active { border:0!important; } .u6872f86bc330512477712e5edee69319 .clearfix:after { content: ""; display: table; clear: both; } .u6872f86bc330512477712e5edee69319 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u6872f86bc330512477712e5edee69319:active , .u6872f86bc330512477712e5edee69319:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u6872f86bc330512477712e5edee69319 .centered-text-area { width: 100%; position: relative ; } .u6872f86bc330512477712e5edee69319 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u6872f86bc330512477712e5edee69319 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u6872f86bc330512477712e5edee69319 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u6872f86bc330512477712e5edee69319:hover .ctaButton { background-color: #34495E!important; } .u6872f86bc330512477712e5edee69319 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u6872f86bc330512477712e5edee69319 .u6872f86bc330512477712e5edee69319-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u6872f86bc330512477712e5edee69319:after { content: ""; display: block; clear: both; } READ: Economic Activity as Reflected in Paintings EssayThe editor accepted Rockwells two finished paintings for covers and also liked his three sketches for future covers. Rockwell had sold everything; his dream was not realized but exceeded. This was the start of a long-term relationship with the Post. His success with the Post made Rockwell more attractive to other major magazines and he began to sell paintings and drawings to Life, Judge, and Leslies. Also in 1916 he married Irene OConnor, a schoolteacher. In 1917, shortly after the United States entered World War I, Rockwell decided to join the navy. He was assigned to the camp newspaper, related Walton, and he was able to continue doing his paintings for the Post and other publications. When the war ended in 1918, Rockwell got an immediate discharge. After the war, besides magazine works Rockwell started advertising illustration. He did work for Jell-O, Willys cars, and Orange Crush soft drinks, among others. Also in 1920, he requested to paint a picture for the Boy Scout calendar. He would continue to provide a picture for the popular calendar for over fifty years. During the 1920s, Rockwell became the Posts top cover artist and his income soared. In 1929 he was divorced from his wife Irene. In 1930, Rockwell married Mary Barstow. They had three sons over the next several years. In 1939,the family moved to a sixty-acre farm in Arlington, Vermont. In 1941, the Milwaukee Art Institute gave Rockwell his first one-man show in a major museum. After President Franklin Roosevelt made his 1941 address to Congress setting out the four essential human freedoms, Rockwell decided to paint images of those freedoms, reported Maynard Good Stoddard of the Saturday Evening Post. With the U. S. entry into World War II. Rockwell created the four paintings during a six-month period in 1942. His Four Freedoms series was published in the Post in 1943. The painting portrayed Freedom of Speech, Freedom of Worship, Freedom of Want, and Freedom from Fear. The pictures became greatly popular and many other publications sent the Post requests to print. Then the federal government took the original paintings on a national tour to sell war bonds. As Ben Hibbs, editor of the Post, noted in Rockwells autobiography, They were viewed by 1,222,000 people in sixteen leading cities and were instrumental in selling $132,992,539 worth of bonds. Then, in 1943, his studio burned to the ground. Rockwell lost some original paintings, drawings, and his exclusive collection of costumes. The family then settled in nearby West Arlington. Over the years Rockwell did illustrations for an ever-widening array of projects. He did commemorative stamps for the postal service. He worked on posters for the Treasury Department, the military, and Hollywood movies. He did mail-order catalogs for Sears and greeting cards for Hallmark. And illustrated books including The Adventures of Tom Sawyer and The Adventures of Huckleberry Finn. In 1953, Rockwell and family moved to Stockbridge, Massachusetts. In the summer of 1959, his wife Mary suffered a heart attack and died. During the 1960s, Rockwell painted portraits of various political figures, including all of the presidential and vice-presidential candidates. Most of these were done for Look magazine. In 1961, he was presented with an honorary Doctor of Fine Arts degree from the University of Massachusetts. That same year he received an award that he especially treasured, wrote Walton. He was given the interfaith Award of the National Conference of Christians and Jews for his Post cover painting of the Golden Rule. Also in 1961, Rockwell married a retired schoolteacher by the name of Molly Punderson. Rockwells last Post cover appeared in December of 1963. Over the years he had done 317 covers. The magazines circulation was shrinking at that time and new management decided to switch to a new format. After Rockwell and the Post parted ways he began a different assignment, painting news pictures for Look. He also started painting for McCalls. In 1969 Rockwell had done a one-man show in New York City. Art critics often were less than flattering toward Rockwells work; if they did not knock him, they ignored him. .u39ea260b9d98dc84f226f7cc7669fcdf , .u39ea260b9d98dc84f226f7cc7669fcdf .postImageUrl , .u39ea260b9d98dc84f226f7cc7669fcdf .centered-text-area { min-height: 80px; position: relative; } .u39ea260b9d98dc84f226f7cc7669fcdf , .u39ea260b9d98dc84f226f7cc7669fcdf:hover , .u39ea260b9d98dc84f226f7cc7669fcdf:visited , .u39ea260b9d98dc84f226f7cc7669fcdf:active { border:0!important; } .u39ea260b9d98dc84f226f7cc7669fcdf .clearfix:after { content: ""; display: table; clear: both; } .u39ea260b9d98dc84f226f7cc7669fcdf { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u39ea260b9d98dc84f226f7cc7669fcdf:active , .u39ea260b9d98dc84f226f7cc7669fcdf:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u39ea260b9d98dc84f226f7cc7669fcdf .centered-text-area { width: 100%; position: relative ; } .u39ea260b9d98dc84f226f7cc7669fcdf .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u39ea260b9d98dc84f226f7cc7669fcdf .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u39ea260b9d98dc84f226f7cc7669fcdf .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u39ea260b9d98dc84f226f7cc7669fcdf:hover .ctaButton { background-color: #34495E!important; } .u39ea260b9d98dc84f226f7cc7669fcdf .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u39ea260b9d98dc84f226f7cc7669fcdf .u39ea260b9d98dc84f226f7cc7669fcdf-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u39ea260b9d98dc84f226f7cc7669fcdf:after { content: ""; display: block; clear: both; } READ: Additive Layer Manufacturing for Aerospace Parts EssayBut the public loved his paintings and many were purchased for prices averaging $20,000. Thomas Buechner wrote in Life, It is difficult for the art world to take the peoples choice very seriously. Rockwell himself said to Walton, I could never be satisfied with just the approval of the critics, and, boy, Ive certainly had to be satisfied without it. In 1975, at the age of 81, Rockwell was still painting, working on his fifty-sixth Boys Scout calendar. In 1976 the city of Stockbridge celebrated a Norman Rockwell Day. On November 8, 1978, Rockwell died in his home in Stockbridge.

Evaluate the Optimum Temperature and PH

Question: Using the correct format write a report on the growth conditions practical you have carried out. The structure of this report will influence your grade. Abstract- one paragraph summary of conclusion and evaluation Method- short, picture can use bullet points Results- table and graph Temperature (oC) Number of yeast cells 5 3487 18 4112 37 5292 37 (acid) 8916 40 7176 50 7308 E.coli using a colour metre Used5 as a blank Temperature (oC) Colour-metre measurement (Abs) 20 0.40 37 0.46 40 0.55 50 0.03 Evaluation- few sentences interpret results Discussion- major of words, what does it tell you, what effect does pH and temp have on e-coli and yeast ( saccharomyces cerevisine) what evidence do you have to support your answer? Compare temperature of yeast and E.coli Conclusion- how to improve experiment what would you do next time? Answer: Abstract The experiment was carried out to evaluate the optimum temperature and pH needed for the optimum growth rate of two organisms selected. The organisms, which were selected, are Saccharomyces serivisiae and Escherichia coli. The result was obtained as the Escherichia coli grown best at 35ËÅ ¡ to 40ËÅ ¡C. where as in case of Saccharomyces cerivisiae, optimum growth was observed at 37ËÅ ¡C with acidic pH condition. Introduction It is a known fact that, every organism has its own set of optimal environmental condition for its optimum growth rate (Pajic-Lijakovic 2015). In case of microorganisms, there are several growth factors, which influence the growth rate of organisms. These factors can be of different physical and chemical factors such as temperature, pH, salt concentration, presence of air, etc. In this lab-report, two organisms are considered to evaluated along with two growth factors or parameters. The organisms, which considered are Escherichia coli and Saccharomyces cerevisiae. The two growth factors, which have been used for the evaluation purpose, are temperature and pH (Myers 2013). Method At first, the materials which are required were autoclaved for the sterilization process After the sterilization, respective growth medium was made and autoclaved. After the completion of the production of the growth media E. coli was inoculated in four media plates and were inoculated at 20ËÅ ¡C, 37ËÅ ¡C, 40ËÅ ¡C and 50ËÅ ¡C respectively. A blank was made for the qualitative purpose and kept at room temperature.(In case of E. coli cells were incubated in liquid culture medium for spectrophotometer reading) Yeast cells were inoculated in six plates and incubated at 5ËÅ ¡C, 18ËÅ ¡C, 37ËÅ ¡ (Normal condition), 37ËÅ ¡C (Acidic), 40ËÅ ¡C and 50ËÅ ¡C respectively. A plate was kept in the room temperature without inoculation to be used as blank. (yeast cells were incubated in solid media plates for colony count) After 24 hours of incubation period yeast culture plates were taken out and cells were counted. (one colony is considered to be one cells) After 30 minutes of incubation, E. coli culture tubes were taken out, the cell growth density was measured using spectrophotometer, and absorbance value was noted. Result After the incubation colony counts were done for the yeast cells and absorbance was noted for the E. coli cells. The results for each cell type are given below in a tabular form. Results for Yeast cells: Temperature (oC) Number of yeast cells 5 3487 18 4112 37 5292 37 (acid) 8916 40 7176 50 7308 Results for E. coli cells: Temperature (oC) Colour-metre measurement (Abs) 20 0.40 37 0.46 40 0.55 50 0.03 Graph for the Yeast cells growth rate: According to the results obtained from the cell count of the yeast cells, it is seen that most of the yeast cells were observed at 37ËÅ ¡C in acidic pH range. Whereas, least amount of cells were observed at 5ËÅ ¡C. Apart from this, at 18ËÅ ¡C, 37ËÅ ¡C (normal), 40ËÅ ¡C and 50ËÅ ¡C cell count was observed as 3487, 4112, 5292, 7176 and 7308 cells respectively. In case of E. coli cells Highest absorbance of was noted at 0.55 nm and lowest absorbance was observed at 50ËÅ ¡C. Along with this, at 20ËÅ ¡C, 37ËÅ ¡C absorbance was noted as 0.40 nm and 0.46 nm respectively. Interpretation From result of the Yeast cell count, it is seen that highest number of yeast cells are obtained in 37ËÅ ¡C acidic plate. From this it can be interpreted that the optimum condition for the Saccharomyces cerivisiae is 37ËÅ ¡C. The pH condition for the growth of Saccharomyces cerivisiae is on the acidic side. Whereas, 5ËÅ ¡C that is low temperature is considered to be adverse condition for the growth of Saccharomyces cerivisiae cells. From the absorbance result of Escherichia coli, it is noted that the highest number of cells were observed at the 40ËÅ ¡C temperature mark. From this temperature, it can be interpreted that the optimum growth temperature for the Escherichia coli cells to grow is about 40ËÅ ¡C. From the result data it can also be interpreted that the least amount of cells were grown at the 50ËÅ ¡C mark. So it can also be said that as the temperature increases cell growth of the Escherichia coli decreases. Conclusion: From this experiment, it can be concluded that the cells have their respective temperature to grow at the optimum rate (Typas 2012). Apart from this, they also have a suitable range of pH range, where their growth rate is maximum. These factors play a crucial role, as the survival and cell division process depends on such factors. In this study the samples were used in the experimental format to obtain the specific optimum temperature and ph for the growth of the selected organism (Winter 2013). But in this case we can assess only a range where the optimum growth has taken place. Further study and experiment is need for the evaluation of exact temperature at which the organism best grows. This aspect is also applied for the pH evaluation process as well. As rate of cell division and cell growth depends on the ph of an environment, it is very important to collect the exact value of these growth factors for a successful evaluation process (Monon 2012). References Monon, J.A.C.Q.U.E.S., 2012. The growth of bacterial cultures.Selected Papers in Molecular Biology by Jacques Monod, p.139. Typas, A., Banzhaf, M., Gross, C.A. and Vollmer, W., 2012. From the regulation of peptidoglycan synthesis to bacterial growth and morphology.Nature Reviews Microbiology,10(2), pp.123-136. Winter, S.E., Winter, M.G., Xavier, M.N., Thiennimitr, P., Poon, V., Keestra, A.M., Laughlin, R.C., Gomez, G., Wu, J., Lawhon, S.D. and Popova, I.E., 2013. Host-derived nitrate boosts growth of E. coli in the inflamed gut.Science,339(6120), pp.708-711. 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