Evaluate the Optimum Temperature and PH

Question: Using the correct format write a report on the growth conditions practical you have carried out. The structure of this report will influence your grade. Abstract- one paragraph summary of conclusion and evaluation Method- short, picture can use bullet points Results- table and graph Temperature (oC) Number of yeast cells 5 3487 18 4112 37 5292 37 (acid) 8916 40 7176 50 7308 E.coli using a colour metre Used5 as a blank Temperature (oC) Colour-metre measurement (Abs) 20 0.40 37 0.46 40 0.55 50 0.03 Evaluation- few sentences interpret results Discussion- major of words, what does it tell you, what effect does pH and temp have on e-coli and yeast ( saccharomyces cerevisine) what evidence do you have to support your answer? Compare temperature of yeast and E.coli Conclusion- how to improve experiment what would you do next time? Answer: Abstract The experiment was carried out to evaluate the optimum temperature and pH needed for the optimum growth rate of two organisms selected. The organisms, which were selected, are Saccharomyces serivisiae and Escherichia coli. The result was obtained as the Escherichia coli grown best at 35ËÅ ¡ to 40ËÅ ¡C. where as in case of Saccharomyces cerivisiae, optimum growth was observed at 37ËÅ ¡C with acidic pH condition. Introduction It is a known fact that, every organism has its own set of optimal environmental condition for its optimum growth rate (Pajic-Lijakovic 2015). In case of microorganisms, there are several growth factors, which influence the growth rate of organisms. These factors can be of different physical and chemical factors such as temperature, pH, salt concentration, presence of air, etc. In this lab-report, two organisms are considered to evaluated along with two growth factors or parameters. The organisms, which considered are Escherichia coli and Saccharomyces cerevisiae. The two growth factors, which have been used for the evaluation purpose, are temperature and pH (Myers 2013). Method At first, the materials which are required were autoclaved for the sterilization process After the sterilization, respective growth medium was made and autoclaved. After the completion of the production of the growth media E. coli was inoculated in four media plates and were inoculated at 20ËÅ ¡C, 37ËÅ ¡C, 40ËÅ ¡C and 50ËÅ ¡C respectively. A blank was made for the qualitative purpose and kept at room temperature.(In case of E. coli cells were incubated in liquid culture medium for spectrophotometer reading) Yeast cells were inoculated in six plates and incubated at 5ËÅ ¡C, 18ËÅ ¡C, 37ËÅ ¡ (Normal condition), 37ËÅ ¡C (Acidic), 40ËÅ ¡C and 50ËÅ ¡C respectively. A plate was kept in the room temperature without inoculation to be used as blank. (yeast cells were incubated in solid media plates for colony count) After 24 hours of incubation period yeast culture plates were taken out and cells were counted. (one colony is considered to be one cells) After 30 minutes of incubation, E. coli culture tubes were taken out, the cell growth density was measured using spectrophotometer, and absorbance value was noted. Result After the incubation colony counts were done for the yeast cells and absorbance was noted for the E. coli cells. The results for each cell type are given below in a tabular form. Results for Yeast cells: Temperature (oC) Number of yeast cells 5 3487 18 4112 37 5292 37 (acid) 8916 40 7176 50 7308 Results for E. coli cells: Temperature (oC) Colour-metre measurement (Abs) 20 0.40 37 0.46 40 0.55 50 0.03 Graph for the Yeast cells growth rate: According to the results obtained from the cell count of the yeast cells, it is seen that most of the yeast cells were observed at 37ËÅ ¡C in acidic pH range. Whereas, least amount of cells were observed at 5ËÅ ¡C. Apart from this, at 18ËÅ ¡C, 37ËÅ ¡C (normal), 40ËÅ ¡C and 50ËÅ ¡C cell count was observed as 3487, 4112, 5292, 7176 and 7308 cells respectively. In case of E. coli cells Highest absorbance of was noted at 0.55 nm and lowest absorbance was observed at 50ËÅ ¡C. Along with this, at 20ËÅ ¡C, 37ËÅ ¡C absorbance was noted as 0.40 nm and 0.46 nm respectively. Interpretation From result of the Yeast cell count, it is seen that highest number of yeast cells are obtained in 37ËÅ ¡C acidic plate. From this it can be interpreted that the optimum condition for the Saccharomyces cerivisiae is 37ËÅ ¡C. The pH condition for the growth of Saccharomyces cerivisiae is on the acidic side. Whereas, 5ËÅ ¡C that is low temperature is considered to be adverse condition for the growth of Saccharomyces cerivisiae cells. From the absorbance result of Escherichia coli, it is noted that the highest number of cells were observed at the 40ËÅ ¡C temperature mark. From this temperature, it can be interpreted that the optimum growth temperature for the Escherichia coli cells to grow is about 40ËÅ ¡C. From the result data it can also be interpreted that the least amount of cells were grown at the 50ËÅ ¡C mark. So it can also be said that as the temperature increases cell growth of the Escherichia coli decreases. Conclusion: From this experiment, it can be concluded that the cells have their respective temperature to grow at the optimum rate (Typas 2012). Apart from this, they also have a suitable range of pH range, where their growth rate is maximum. These factors play a crucial role, as the survival and cell division process depends on such factors. In this study the samples were used in the experimental format to obtain the specific optimum temperature and ph for the growth of the selected organism (Winter 2013). But in this case we can assess only a range where the optimum growth has taken place. Further study and experiment is need for the evaluation of exact temperature at which the organism best grows. This aspect is also applied for the pH evaluation process as well. As rate of cell division and cell growth depends on the ph of an environment, it is very important to collect the exact value of these growth factors for a successful evaluation process (Monon 2012). References Monon, J.A.C.Q.U.E.S., 2012. The growth of bacterial cultures.Selected Papers in Molecular Biology by Jacques Monod, p.139. Typas, A., Banzhaf, M., Gross, C.A. and Vollmer, W., 2012. From the regulation of peptidoglycan synthesis to bacterial growth and morphology.Nature Reviews Microbiology,10(2), pp.123-136. Winter, S.E., Winter, M.G., Xavier, M.N., Thiennimitr, P., Poon, V., Keestra, A.M., Laughlin, R.C., Gomez, G., Wu, J., Lawhon, S.D. and Popova, I.E., 2013. Host-derived nitrate boosts growth of E. coli in the inflamed gut.Science,339(6120), pp.708-711. Pajic-Lijakovic, I., Levic, S., HadnaÄ‘ev, M., Stevanovic-Dajic, Z., Radosevic, R., Nedovic, V. and Bugarski, B., 2015. Structural changes of Ca-alginate beads caused by immobilized yeast cell growth.Biochemical Engineering Journal,103, pp.32-38. Myers, J.A., Curtis, B.S. and Curtis, W.R., 2013. Improving accuracy of cell and chromophore concentration measurements using optical density.BMC biophysics,6(1), p.4.